Abstract From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers Contraceptives in the United States.The swabs were placed in a virus transport medium and transported to the laboratory on ice packs.At the laboratory, the samples were pooled with each pool consisting of five samples.All pools (n = 50) were screened for the presence of avian influenza virus (AIV) using a real time reverse transcription-polymerase chain reaction (rRT-PCR); one of the pools was found positive.All five samples in this pool were tested individually by rRT-PCR; one sample from a bald eagle was found positive.
This sample was inoculated in embryonated chicken eggs for virus isolation and a hemagglutinating virus was isolated.Complete genome sequencing of the isolate revealed a mixed infection with H1N1 and H2N1 subtypes.Further analysis revealed that the PB1-F2 gene sequence of H1N1 virus had the LIVER MD N66S virulence-associated substitution.Further studies on ecology and epidemiology of AIV in raptors are needed to help understand their role in the maintenance and evolution of AIV.